Blog. 18 December Prezi Awards The best presentations have arrived. 5 December Do this, not that: Keynote speech. 28 November Wady i Zalety Klonowania Idea 1. Idea 2. Rozmnażanie bezpłciowe – naturalne klonowanie. Klonowanie zachodzi przez: Klonowanie DNA. KLONOWANIE Klony DNA służą do: Co to jest klonowanie? Klonowanie – tworzenie genetycznej kopii fragmentu DNA, komórki lub organizmu.
|Published (Last):||13 November 2014|
|PDF File Size:||18.85 Mb|
|ePub File Size:||10.18 Mb|
|Price:||Free* [*Free Regsitration Required]|
Przegląd: Klonowanie DNA
Because of this, the newly made protein needs to be purified separated from the other proteins and macromolecules before it can be used. DNA cloning can be used to make human proteins with biomedical applications, such as the insulin mentioned above. This is not a useful plasmid. Insert the plasmid into bacteria. The bacteria are given a heat shock, which “encourages” them to take up a plasmid.
So you might have an overhang over there, you might have an overhang over there. It’s going to take in the plasmid. So if you have a bacteria, you have a bacteria right over here, it has its existing DNA, so this is its existing genetic material right over there, let me label this. In some cases, plasmids are directly used for practical purposes. And so the plasmid that we’re placing in might have complimentary base pairs over the overhangs, which will allow it easier, it will become easier klknowanie them to react with each other if they have these overhangs.
Transformation is a key step in DNA cloning. It klonwoanie nutrients, and so you could say, okay well put this here and then a bunch of bacteria will just grow. The copies are often made in bacteria. And you actually wouldn’t be able to see it visually but there is E. Using a carefully chosen restriction enzyme, we digest:.
Several colonies are checked to identify one with the dnz plasmid e. You’ve used the restriction enzymes to cut out your gene and then what you wanna do is you wanna paste it into what we’ll call a plasmid. It occurs after restriction digest and ligation and transfers newly made plasmids to bacteria.
How can pieces of DNA from different sources be joined together? And so what you do is in your nutrients you grew nutrients plus antibiotics, klonowwanie an antibiotic. So now you have a gene for antibiotic resistance here, and so only the bacteria, and I think it’s amazing that klonoowanie as humanity are able to do these types of things, but now only the bacteria that have taken up the plasmid will have that antibiotic resistance.
So gene to clone. They recognize specific sequences and then we can figure out well which restriction enzyme should we use to cut out different pieces of DNA, but we have gotten to that point as a civilization. The bacteria can then be lysed split open to release the protein.
Selekcja i transformacja u bakterii. Specially prepared klonodanie are mixed with DNA e. There are a variety of different techniques used for protein purification.
Klonowanie by Adriana Szaposznikow on Prezi
Klonowanie i rekombinacja DNA. How does that work? Thus, all of the bacteria are placed on an antibiotic plate to select for ones that took up a plasmid. It’s identical copies of a piece of DNA.
Klonowanie i rekombinacja DNA.
If a plasmid contains the right control sequences, bacteria can be induced to express the gene it contains when a chemical signal is added. What is the point of making many copies of a DNA sequence in a plasmid?
Now the next question, and I’m over simplifying things fairly dramatically is well you now have a bunch of bacteria that have a bunch of copies of that gene, how do you make use of it? And the typical shock is a heat shock.
The bacteria that make colonies should all contain a plasmid which provides antibiotic resistance. So there we go. In some cases, we need lots of DNA copies to conduct experiments or build new plasmids. Selekcja i transformacja u bakterii. And the technique that’s typically done is giving some type of a shock to the system that makes the bacteria take up the plasmids.
But maybe this colony is formed by an initial bacteria or a set of bacteria that did not take up the plasmid so it won’t contain the actual gene in question.
You might have a little bit left over on either side but essentially you have cut out the gene.